Table 2. Quantification of amplified transcripts from DNA repair genes and TERT in skeletal muscle cells of mice treated by CR, H2O2, ART, SNP, ARG, or AL.
(A) The normalization of quantified transcripts of DNA repair genes and TERT by a specific target gene vs the reference gene GAPDH. (B) The fold changes of quantified transcripts of DNA repair genes and TERT in treated mice vs AL mice.
| Sample | BRCA2 /GAPDH |
RB /GAPDH |
MYC /GAPDH |
RAD50 /GAPDH |
RAD51 /GAPDH |
TERT /GAPDH |
|---|---|---|---|---|---|---|
| CR | 6.48E−05 | 3.21E−04 | 6.40E−05 | 3.33E−05 | 9.68E−05 | 1.64E−05 |
| H2O2 | 9.87E−05 | 1.82E−03 | 4.62E−03 | 2.95E−04 | 1.39E−04 | 3.07E−05 |
| ART | 9.29E−05 | 1.48E−03 | 1.56E−03 | 5.79E−04 | 1.87E−04 | 4.14E−05 |
| SNP | 1.36E−04 | 1.64E−03 | 3.90E−04 | 3.31E−04 | 1.83E−04 | 1.94E−05 |
| ARG | 6.51E−05 | 1.17E−03 | 2.71E−04 | 3.02E−04 | 1.50E−04 | 7.36E−06 |
| AL | 1.13E−04 | 3.00E−04 | 2.83E−04 | 2.33E−04 | 1.29E−04 | 2.87E−05 |
Notes.
The RT-PCR was performed after daily injection for three days into mouse skeletal muscle by 260 µM ART, 67 µM SNP, 5.7 mM ARG in the dose of 50 µl volume/20 g body weight, or one injection by 200 µM H2O2 (50 µl/20 g), and sampling after last injection for six hours. The ages of all mice used are four-month-old, among which CR mice have one-month AL and three-month CR treatment.