Figure 3.
Rates of hydrolysis of analogues 9 and 10B at pH 1.4 at 37 °C, as monitored by HPLC. Hydrolysis of 7.5 mM analogue 9 or 10B in KCl/HCl buffer at pH 1.4 and 37 °C was recorded for 5 days at 7–17 h intervals. (A) HPLC chromatograms of analogue 9 and hydrolysates at t = 6, 52, 216 h. (B) Kinetics of acidic hydrolysis of analogue 9 (t1/2 = 65 h) and time-dependent formation of degradation products. (C) Kinetics of acidic hydrolysis of analogue 10B (t1/2 = 8 h).