Table 1. Relative Hydrolysis [%] of Analogues 8–14 by Human ecto-Nucleotidasesa.
| analogues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
|
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| human ecto-nucleotidases | 8 | 9 | 10A | 10B | 11A | 11B | 12A | 12B | 13 | 14 |
| NTPDase1 | 2.6 | 0.5 | 1.0 | 0.4 | 5.5 | 3.2 | 0.7 | ND | 1.3 | ND |
| NTPDase2 | 1.1 | 0.5 | 1.8 | 0.9 | 1.3 | 1.4 | 1.5 | ND | 2.4 | 1.7 |
| NTPDase3 | 2.6 | ND | 4.4 | ND | 2.6 | 3.1 | 0.3 | ND | ND | ND |
| NTPDase8 | 5.2 | ND | 1.7 | ND | 4.4 | 4.0 | ND | 1.8 | 0.1 | ND |
| NPP1 | 6.2 | 5.8 | 4.0 | 3.4 | 5.3 | 3.3 | 0.7 | 4.0 | 3.9 | 3.3 |
| NPP3 | 0.5 | 1.9 | 10.0 | 3.2 | ND | ND | 5.0 | ND | ND | 1.8 |
a
The ATP and ADP analogues 8–14 (100 μM) were used as sub-strates of the ecto-nucleotidases identified in the left column. The relative hydrolysis rates vs 100 μM ATP (set at 100%) were: 403 ± 40, 1006 ± 60, 533 ± 42, 229 ± 20 [nmol Pi·min−1·mg protein−1] for NTPDase1,2,3,8, respectively, and 619 and 245 [nmol nucl·min−1·mg protein−1] for NPP1,3, respectively. ND = not detectable.