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. 2015 Mar;75:91–99. doi: 10.1016/j.nbd.2014.12.011

Fig. 2.

Fig. 2

UCM increase frataxin levels.

(A) To detect frataxin precursor accumulation, 293 Flp-In cells stably expressing frataxin1 -210 were treated for 24 hrs with 10 μM of the indicated UCM or 10 μM MG132 (MG). Total cell extracts were resolved on SDS–PAGE and analyzed with anti-frataxin antibody, or anti-tubulin, as a loading control. Pre: precursor; tub: tubulin. (B) To detect mature frataxin accumulation, 293 Flp-In cells stably expressing frataxin1 -210 were treated for 72 hrs with 10 μM of the indicated UCM. Total cell extracts were resolved on SDS–PAGE and analyzed with anti-frataxin antibody, or anti-tubulin, as a loading control. Pre: precursor, int: intermediate, mat: mature; tub: tubulin.