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. 2015 Mar;75:91–99. doi: 10.1016/j.nbd.2014.12.011

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — UCM interact with frataxin.

Fluorescence studies of the interaction of 2 μM recombinant frataxin precursor with different concentration of the indicated UCM (ligand). The graphs represent the fractional loss of the protein fluorescence intensity (ΔF/F₀), in the presence of different concentration of ligand, versus the ratio between ligand and frataxin. The interaction with UCM53 was also analyzed in the case of frataxin precursor previously denaturated in 3 M guanidinium hydrochloride (upper left panel, black square symbols). The half-saturation binding constant, L_1/2 (μM), of frataxin precursor with UCM53, UCM108 and UCM72 is indicated in each corresponding panel.