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. Author manuscript; available in PMC: 2015 Mar 13.
Published in final edited form as: Bone. 2013 Dec 4;59:229–234. doi: 10.1016/j.bone.2013.11.026

Figure 3.

Figure 3

Tracer transport through the lacunar-canalicular system was assessed using confocal microscopy to identify osteocyte lacunae considered labeled in both loaded and unloaded tibiae. (a) A typical grayscale image of cancellous bone of the metaphysis. The trabecula shown is surrounded by bone marrow, and the fully visible osteocytes are indicated with arrows. (b) The same image after thresholding; labeled osteocyte lacunae are marked with arrows. (c) Higher magnification of the image in (a) showing two osteocytes, and (d) the same image after thresholding. The arrows in (d) indicate that both osteocytes satisfied the criteria to be counted as labeled (osteocyte lacunae presenting at least 80% of the lacunar space stained or 80% of the periphery of the lacuna stained).