Fig 3. Adult Schistosoma mansoni motility is reduced in the presence of 7-keto-sempervirol.

World Health Organisation motility metrics were used for scoring adult S. mansoni male and female worm phenotypes (5 worm pairs/ml) co-cultured (37°C and 5% CO₂) with 7-keto-sempervirol (100 μM final concentration) for 24 hr, 48 hr and 72 hr. Phenotypes derived from a control group (media containing 1% v/v DMSO) of male and female worms cultivated for 24 hr (worm pairs cultured for 48 hr or 72 hr were identical to the 24 hr worms, S2 Fig.) is also illustrated. Mean motility values are indicated as histograms (n = 5 replicates/time-point) and the error bars represent the standard deviation of the mean (SD). Different letters indicate a significant difference between the mean phenotypic scores calculated by ANOVA and Tukey’s post-hoc tests.