Fig 4. The diterpenoid 7-keto-sempervirol induces surface tegumental damage in adult Schistosoma mansoni males.

Scanning Electron Microscopic (SEM) images of adult S. mansoni male worms cultured for 72 hr at 37°C and 5% CO₂ in the presence (100 μM) or absence (1% v/v DMSO) of 7-keto-sempervirol. A) (left) 1.00K x magnification of a control (1% v/v DMSO) adult male surface (anterior end) and corresponding (right) 6.00K x magnification of normal tubercles. B) (left) 1.00K x magnification of a 7-keto-sempervirol treated (100 μM), adult male surface (anterior end) and enlarged (right) 9.00K x magnification of two indicated (black boxes) areas of tegumental damage. Holes in the surface tegument/tubercles are indicated by white arrows. These images are representative of 5 adult worms/condition.