Fig 5. Schistosoma mansoni egg-shell formation is inhibited by 7-keto-sempervirol.

Adult S. mansoni female worms were cultured (+/- 100 μM 7-keto-sempervirol) for 24 hr at 37°C and 5% CO₂. A) 40x laser scanning confocal microscopic image of an egg derived from a control female (1% v/v DMSO) demonstrating regular auto-fluorescence and a well-formed lateral spine (normal architecture of a S. mansoni egg). B) 40x laser scanning confocal microscopic image of an egg derived from one adult female worm treated with 100 μM 7-keto-sempervirol. Images are representative of typical in utero egg phenotypes obtained from 5 worms/condition.