Fig 6. Schistosoma mansoni oviposition is inhibited by 7-keto-sempervirol.

Adult worm pairs (5 pairs/ml) were co-cultured (37 ^oC and 5% CO₂) with 1% v/v DMSO (DMSO control), 10 μM 7-keto-sempervirol or 100 μM 7-keto-sempervirol for 72 hr. A) Bar charts represent the mean percentage of abnormal eggs (lacking a fully formed lateral spine) produced after 72 hr (n = 5 replicates/treatment). Error bars represent the standard deviation of the mean (SD). After 72 hr, a total of 68.4 +/- 74.4 eggs (normal and abnormal) were enumerated in the worm cultures containing 1% DMSO, 73.2 +/- 46.4 eggs (normal and abnormal) were observed in the cultures containing 10 μM 7-keto-sempervirol and zero eggs were counted in the wells containing 100 μM 7-keto-sempervirol. Student’s t-test indicates that a significant difference exists between the percentage of abnormal eggs laid between the 1% v/v DMSO and 10 μM 7-keto-sempervirol treatments (p = 0.01). B) Fluorescent images of representative eggs deposited in vitro demonstrate normal egg architecture in the DMSO control wells (DMSO control) and abnormal egg architecture in the 10 μM 7-keto-sempervirol wells (7-keto-sempervirol).