Fig. 3.

Induced-mitophagy can be used to measure differential autophagy kinetics in different cell lines. (A) 3T3-SA-YFP-Parkin, Saos2 YFP-Parkin and SVEC YFP-Parkin cells were treated with 1 μM antimycin A + 1 μM oligomycin for the indicated time periods. Cell lysates were analyzed by Western blot using the MitoProfile Membrane integrity WB Antibody Cocktail, and an anti-LC3B antibody. Actin was used as a loading control. (B) Protein quantification from three independent experiments was determined by ImageJ software using the intensity of the band and expressed as mitochondria protein to actin ratio (%). ^∗,∗∗Indicate significant differences between 3T3-YFP-Parkin and Saos-2 YFP-Parkin (^∗p < 0.05; ^∗∗p < 0.001) and $ indicates significant difference between SVEC YFP-Parkin and Saos-2 YFP-Parkin. ^$p < 0.05; ^$$p < 0.01.