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. 2015 Feb 21;13:67. doi: 10.1186/s12967-015-0422-3

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© Lu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ASC-CM suppresses activation of pro-apoptotic pathways in HPAEC. HPAEC were treated with vehicle control, NHDF-CM, and hASC-CM, and then stimulated with indicated concentration of TNFα (4 h). Cell lysates were analyzed with antibodies to cleaved caspase 3, Bad, phospho-Bad, Bim, and Bcl2. β-actin staining was used as loading control.