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. 2015 Feb 21;13:67. doi: 10.1186/s12967-015-0422-3

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© Lu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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mASC and mASC-CM effect on neutrophil numbers and activity. Total WBC (T, grey) and PMN (N, white) counts in BALF were significantly reduced by mASC (A), but not mASC-CM (B). The ratio between macrophages (M), lymphocytes (L) and neutrophils (N) in BALF of LPS-treated mice was not significantly affected by either experimental treatment. N = 5-6 animals per group. (C) WBC from BALF of LPS-challenged mice (N = 6) were subjected to vehicle (LPC cn) or 1 μg/ml LPS stimulation (LPS st). In parallel, WBC from BALF of LPS/ASC-CM-treated mice (N = 6) were subjected to vehicle control (LPS/CM cn) or LPS stimulation (LPS/CM st). LPS-induced ROS generation by WBC from BALF of LPS/ASC-CM mice was significantly lower comparing to WBC from BALF of LPS/media mice. T-test was used to analyze the significance of differences between groups.