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. 2015 Mar 7;15:29. doi: 10.1186/s12935-015-0177-1

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© Cheng et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Plectin-knockdown Chang liver cells appears to have increased Rac1-GTPase activity. Total lysate separately prepared from mock cells and plectin siRNA-transfected cells were applied to Rac1-GTPase pull-down assay and Western blotting assay with anti-Rac1 and anti-plectin antibodies. (a) The result showed that the Rac1-GTPase level was increased in plectin-knockdown cells. (b) The results indicated the quantification of Rac1-GTPase level. Values are presented as mean ± standard error obtained from three independent experiments, * indicates p < 0.05.