Table 2. Summary of NGS counts and library coverage.

Library Name	NGS Counts	Primary Coverage	Secondary Coverage
Naïve: Following Transformation in E.coli	168,627	99.45% 1	99.69% 2
25°C: After Permissive Growth in Yeast	63,393	86.35% 1	89.13% 3
37°C: After Restrictive Growth in Yeast	158,782	48.31% 1	N/A

¹Based on the observation that a sequence motif is detected at least twice in a particular NGS run.

²Based on the observation that a sequence motif is detected at least twice in the Naïve, 25°C or 37°C data set.

³Based on the observation that a sequence motif is detected at least twice in either the 25°C or 37°C data set.