Fig 2. miR-185 regulates the expression of CaMKIIδ, NCX1, and NFATC3 in cultured NRVMs.
(A-C) qRT-PCR analyses of Camk2d, Ncx1 and Nfatc3 expression after transfection with miR-185 or NC. (D-F) miR-185 transfected NRVMs were lysed and analyzed by western blotting using antibodies against the proteins of interest. GAPDH or α-tubulin was used as a loading control. Representative western blots (upper) and quantified western blots (bottom). Data represent the mean ± SEM; *P < 0.05, **P < 0.001, N = 4.
