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. 2015 Mar 13;10(3):e0122509. doi: 10.1371/journal.pone.0122509

Fig 3. miR-185 directly targets 3′-UTR of Camk2d, Ncx1, and Nfatc3.

Fig 3

(A) Schematic diagram of the pmirGLO chimeric vector indicating where the exact complement target sequences for miR-185 or mutant sequences were cloned into the 3′-UTR of the luciferase gene. (B-D) The relative positions of predicted binding sites for mouse miR-185 in the 3′-UTR of the target mRNAs (upper) and quantitative analysis of luciferase activity of the reporter constructs (bottom). HEK-293 cells were transfected with miR-185 in addition to the reporter constructs with WT or mutated 3′-UTR. After 72 h following transfection, cells were lysed for dual luciferase assay. Hsa, human; Mmu, mouse; Rno, rat; Ptr, chimpanzee. Data represent the mean ± SEM; **P < 0.001, or NS (not significant), N = 3.