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. 2015 Mar 13;9(3):e0003552. doi: 10.1371/journal.pntd.0003552

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© 2015 Silva-Álvarez et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — The lipid composition of rEgAgB8 subunits prior to delipidation (Pre), as well as rEgAgB8 subjected to RP-HPLC (Post) were analysed by TLC, in parallel with standards for neutral and polar lipids. Lipid bands were visualised using CuSO₄/H₃PO₄ and identified by comparison with the standards. (A) The lipids extracted from E. coli grown under the same culture conditions is shown for comparison. TLCs from neutral and polar lipids were undertaken separately. (B) The lipid moiety of recombinant subunits were analysed by TLC using double development. The lipid fraction of rEgAgB8 pre-HPLC contained mainly polar lipids (PE and CL), which were successfully removed by the RP-HPLC method. PC: phosphatidylcholine; PS: phosphatidylserine; PI: phosphatidylinositol; CL: cardiolipin; PE: phosphatidylethanolamine; Cho: cholesterol; FA: free fatty acids; DAG: diacylglycerols; TAG: triacylglycerols; SE: sterol esters.