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. 2015 Mar 13;9(3):e0003552. doi: 10.1371/journal.pntd.0003552

Fig 4. SEC analysis of lipid-free EgAgB subunits.

Fig 4

Analysis of EgAgB lipid-free subunits by SEC was undertaken on a Superdex 200 column employing a flow rate of 0.5 mL/min. Elution profiles of lipid-free EgAgB8/2 and EgAgB8/3 were followed at 215 nm. Both subunits showed a well-defined peak, with a molecular weight estimation of 62 kDa for EgAgB8/2 and 38 kDa for EgAgB8/3 according to standard proteins analysed under the same conditions (a: BSA, 66 kDa; b:carbonic anhydrase, 29 kDa; and c: cytochrome c, 12 kDa).