Fig 2. Western blot and IHC Analysis of TLR Expression in 661W cells.

661W cells were either left untreated or challenged with the indicated TLR ligands (as described in Fig. 1 legend) for 8h. Immunostaining was performed using specific antibodies to access TLR expression (A). In a separate experiment, cells were lysed using RIPA buffer and analyzed for TLRs expression by Western blot analysis using specific anti-TLR antibodies, with β-actin as a loading control (B). Band intensity was quantified by using Image J and presented as the relative band intensity of TLR vs. β-actin (C). Data points and error bars represent mean ± SD from two independent experiments.