Fig 4. Effect of TLR agonists on secretion of inflammatory mediators in 661W cells.

661W cells were challenged with the indicated TLR ligands for 8h and the secretion of inflammatory mediators was measured in conditioned media by ELISA. Data points and error bars represents mean ± SD of duplicates from three independent experiments. Statistical analysis was performed using one-way ANOVA (*, p<0.05; **, p<0.005; ***, p<0.005), for comparisons of control versus stimulated cells.