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. 2015 Mar 13;10(3):e0120257. doi: 10.1371/journal.pone.0120257

Fig 3. Effect of 24 hr exposure to palmitate and oleate on human BMMSC mitochondrial membrane potential.

Fig 3

A) Images of tetramethylrhodamine methyl ester (TMRM) stained bone marrow mesenchymal stem cells (BMMSCs) treated for 24 hr with indicated treatments. B) Relative TMRM levels. BMMSCs were treated for 24 hr with 0.55 mM bovine serum albumin (BSA) alone or palmitate and/or oleate bound to 0.55 mM albumin. TMRM and Hoechst stain were added to the medium to measure mitochondrial membrane potential and stain nuclei, respectively, and images were taken. All fatty acid treated groups were also treated with media supplemented with 0.55 mM albumin in addition to the type and amount of fatty acid indicated in the figures. 4 separate observations. Values are shown as the mean ± SEM. * Significantly different from the BSA group.