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. 2015 Mar 13;9(3):e0003578. doi: 10.1371/journal.pntd.0003578

Fig 4. A series of operations of 18S-rRNA RT-LAMP screening test.

Fig 4

Two hundred μl of blood were diluted in 1,800 μl of lysis buffer (10× dilution), then 10 μl of lysed blood were transferred into a Dry LAMP tube with 15 μl of reaction buffer within 5 minutes. After a 45-min incubation at 60°C using a portable battery-supplied heat block, the LAMP reaction was evaluated by a portable reaction detector.