Table 1. Multiplex detection of miRNAs using pincer probe or stem-loop real-time RT-PCR.
| MiRNA target | Pincer probe RT-PCR | Stem-loop RT-PCR | ||||
|---|---|---|---|---|---|---|
| Liver* | Heart | Lung* | Liver | Heart | Lung | |
| miR-16 | 24.3 | 21.8 | 23.7 | 24.0 | 22.2 | 22.6 |
| miR-20 | 25.7 | 24.2 | 24.2 | 24.2 | 23.8 | 24.2 |
| miR-21 | 24.8 | 26.6 | 21.9 | 23.9 | 24.9 | 22.1 |
| miR-22 | 28.5 | 26.0 | 29.7 | 27.9 | 26.2 | 28.6 |
| Let-7a | 27.2 | 24.6 | 24.8 | 25.2 | 23.6 | 24.6 |
| Let-7b | 27.8 | 27.9 | 25.6 | 25.8 | 26.8 | 24.7 |
| Let-7c | 25.2 | 22.9 | 23.4 | 24.3 | 23.8 | 22.8 |
| Let-7d | 29.8 | 27.2 | 29.1 | 29.3 | 26.7 | 28.4 |
| Let-7e | 34.4 | 32.5 | 30.1 | 32.4 | 31.1 | 29.0 |
*Paired T-test was performed to show the difference between the pincer probe method and the stem-loop RT-PCR method. The signal of the pincer probe method is significantly lower than the stem-loop RT-PCR for the liver tissue (p-value < 0.01) and lung tissue (p-value < 0.01).