Table 2. Discrimination between the mature and the precursor miRNAs using pincer probe or linear real-time RT-PCR.
| MiRNA target | Pincer probe RT-PCR | Linear RT-PCR | ||||
|---|---|---|---|---|---|---|
| Precursor* | Mature* | Delta Cq | Precursor | Mature | Delta Cq | |
| mir-133 | 36.1 | 28.0 | 8.1 | 34.1 | 30.0 | 4.1 |
| mir-122 | 38.5 | 29.1 | 9.4 | 36.5 | 33.4 | 3.1 |
| mir-155 | 34.2 | 28.5 | 5.7 | 33.2 | 29.2 | 4.0 |
| Let-7a | 37.7 | 28.7 | 9.0 | 35.2 | 36.5 | -1.3 |
| Let-7b | 39.4 | 29.3 | 10.1 | 37.5 | 32.5 | 5.0 |
| Let-7c | 40.0 | 30.2 | 9.8 | 37.0 | 35.0 | 2.0 |
| Let-7d | 35.5 | 28.1 | 7.4 | 33.0 | 34.0 | -1.0 |
| Let-7e | 37.5 | 28.0 | 9.5 | 34.5 | 30.2 | 4.3 |
| Let-7f | 39.5 | 30.1 | 9.4 | 37.5 | 30.1 | 7.4 |
| Let-7g | 37.0 | 28.5 | 8.5 | 34.2 | 29.0 | 5.2 |
| Let-7i | 38.2 | 28.8 | 9.4 | 34.0 | 32.6 | 1.4 |
*Paired T-test was performed to show the difference between the pincer probe method and the linear RT-PCR method. The signal of the pincer probe method is significantly lower than the linear RT-PCR for the mature miRNAs (p-value < 0.01) and significant higher for the precursors (p-value < 0.01).