. Author manuscript; available in PMC: 2016 May 1.

Published in final edited form as: J Comp Neurol. 2015 Feb 17;523(7):1054–1072. doi: 10.1002/cne.23707

Table 3. Stereological parameters for estimates of pyramidal neuron counts and volumes.

Sampling Grid Area (μm²)		4,410,000
Counting Frame Area (μm²)		2,025
Disector Height (μm)		30
Guard Zone Height (μm)		10
Sections Sampled (Avg. #)		12
Mean Measured Thickness (μm)	200 μm cut thickness	179
	500 μm cut thickness	418
	600 μm cut thickness	550
Optical Fractionator Sample (Avg. #)		324
Optical Fractionator CE (Gundersen, M = 1)		< 0.07
Nucleator Sample (Avg. #)		81
Nucleator CE		<0.02

Mean measured thickness is presented for tissue that was originally sectioned at 200, 500, and 600 μms.