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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Anal Bioanal Chem. 2014 Dec 9;407(8):2265–2271. doi: 10.1007/s00216-014-8376-5

Figure 1.

Figure 1

Ion maps of cholesterol ([M-H2O+H+]+) before and after the optimization of the electrospray flow rate and spray voltage. Flow rate and spray voltage were decreased in order to improve signal abundance. The tissue boundary is illustrated using the dotted line.