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The optical focus diameter (300 μm) and the desorption diameter (150 μm) on tissue illustrates the semi- Gaussian distribution of the laser beam (a). Areas ablated with a spot-to-spot distances of 100 μm (b), 30 μm (c), and 10 μm (d) are depicted. The volume of each voxel was calculated considering a 10-μm thick tissue section.
