Figure 2.
(A) - mass spectrum acquired from a single pixel in the image taken from an area of mouse cerebellum, (B) – MS ion images from the same area for 3 ions: red – m/z 14127 (myelin basic protein, isoform 8), green – m/z 13804 (histone H2B), blue – m/z 11347 (histone H4) represented as one combined RGB image. The image is overlaid with the optical microscope image of the same area that was stained with H&E following MS image acquisition. The MS imaging field is 500×500 μm scanned by a laser raster of 2.5 μm step size and laser spot diameter 1 μm on target.