. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: Pharm Res. 2014 Oct 18;32(4):1395–1406. doi: 10.1007/s11095-014-1542-9

Table IV.

Binding Constants (Ka) obtained from equilibrium dialysis and fluorescence quenching and Stern Volmer constant (Ksv) obtained from fluorescence quenching at room temperature. Concentration of drugs used in both studies ranged from 0.7 mM - 0.025 mM

Formulation	Ka (equilibrium dialysis with HSA) (M⁻¹ ± SEM)^*	Ksv (fluorescence quenching) with HSA (M⁻¹)^***	Ka (fluoroscence quenching with AGP) (M⁻¹)^***	Ksv (fluorescence quenching) with AGP (M⁻¹)^***
JS-K	3.4 × 10³ ± 2.2 Ns^** = 2.2; R² = 0.84	1.2 × 10³ R² = 0.59	15 × 10³ R² = 0.77	3 × 10³ R² = 0.91
P123/JS-K	3.0 × 10³ ± 3.6 Ns^** = 1.9; R² = 0.89	2.2 × 10³ R² = 0.805	3.5 × 10³ R² = 0.99	2 × 10³ R² = 0.67

Average and standard error of mean (SEM) of at least three independent experiments.

^**

Ns=No. of binding sites.

^***

Average of at least three independent experiments.

R² = goodness of fit