Skip to main content
. 2015 Mar 13;10(3):e0120250. doi: 10.1371/journal.pone.0120250

Fig 2. Ypk3, but not Sch9, is required for Rps6 phosphorylation at Ser232/233.

Fig 2

A) The indicated strains in the BY4742 background were grown in synthetic complete medium for 30 h (stationary phase) and then shifted to fresh medium (+) for 1 h. Total lysates were analyzed by Immunoblot using anti-phospho-S6 antibody. B) Immunoblot analysis of Rps6 phosphorylation in WT and Sch9-deficient cells in the TB50a background. Cells were shifted from nitrogen-free medium to SC medium as in Fig. 1E and aliquots were taken at the indicated time points. C) Immunoblot analysis of Rps6 phosphorylation in WT and ypk3Δ cells. Cells were shifted from nitrogen-free medium to SC medium as in Fig. 1E and aliquots were taken at the indicated time points.