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. 2015 Mar 13;9(3):e0003468. doi: 10.1371/journal.pntd.0003468

Fig 4. Comparison of DENV replication in A549 cells detected by RT-PCR.

Fig 4

A549 cells were infected with representative DENV strains at an MOI = 0.1 for 1 hour. After washing, cells were incubated for the time period indicated (12, 24, 48, or 72 h) and cell supernatants were harvested for quantification of viral genomes by real-time RT-PCR as described in Materials and Methods. Experiments were performed in triplicate. Results shown are representative of two independent experiments.