Fig 7. DENV2 16681 blocks STAT1 phosphorylation but can’t inhibit production of IFN-α in primary myeloid dendritic cells.

Dendritic cells from representative were mock-infected or infected with DENV-1 16681 at an MOI of 5 for twenty-four hours. (A) Cells were stimulated for 30 min. with IFN-β (500 U/ml) and co-stained with anti-pSTAT1 Alexa 647- and anti-dengue prM Alexa 488-conjugated antibodies. Cell fluorescence was measured on a BD FACS Calibur and data analysis was conducted using FlowJo software. (B) The concentrations of IFN-α in the supernatants were quantified by ELISA. Results shown for flow cytometry are representative of two human and two Rhesus macaque samples. Results shown for IFN-α production were done in triplicate and represent the values obtained from two Rhesus macaques.