Representative bands for Western analysis of phospho(Ser9)-GSK3β, total GSK3β, and actin (used as loading control) are shown for soleus muscle of ambulatory (amb), hind limb suspended (HS) and reloaded (HSRE) rats at 6 and 32 months (A). Quantification of Western analysis is depicted for phospho- GSK3β (B), total GSK3β (B) and the ratio of phospho to total GSK3β (C) for ambulatory (black bars), hind limb suspended (HS, white bars), and reloaded (HSRE, grey bars) rats at 6 and 32 months (n=8–10). Values are means ± SE; * indicates a main effect for age and # indicates a main effect for HS; p<0.05.