Table 1. Bumble bees used in amplicon sequencing and ARISA, and their geographic origins.
| ARISA ID | Pyrosequencing sample ID | Bombus species | State/Province | Country | Latitude | Longitude | Field ID (if available) |
|---|---|---|---|---|---|---|---|
| ATRATUS | B. atratus | Boyaca | Columbia | 5.64 | -72.9 | ||
| ATRATUS | B. atratus | Boyaca | Columbia | 5.64 | -72.9 | ||
| BOHEMICUS | B. bohemicus | Uppland | Sweden | 60.48 | 17.62 | ||
| BOHEMICUS | B. bohemicus | Uppland | Sweden | 60.48 | 17.62 | ||
| BOHEMICUS | B. bohemicus | Uppland | Sweden | 60.48 | 17.62 | ||
| CONVEXUS | B. convexus | Sichuan | China | 32.79 | 102.54 | ||
| CONVEXUS | B. convexus | Sichuan | China | 32.79 | 102.54 | ||
| CONVEXUS | B. convexus | Sichuan | China | 32.79 | 102.54 | ||
| B.FR7 | FRATERNUS_FL | B. fraternus | Florida | USA | 30.13 | -83.35 | FL11.0011 |
| B.FR9 | FRATERNUS_FL | B. fraternus | Florida | USA | 30.13 | -83.35 | FL11.0009 |
| B.FR10 | B. fraternus | Florida | USA | 30.13 | -83.35 | FL11.0010 | |
| B.FR2 | B. fraternus | Georgia | USA | 31.35 | -82.02 | GA11.0031 | |
| B.FR3 | B. fraternus | Georgia | USA | 31.35 | -82.02 | GA11.0028 | |
| B.FR4 | B. fraternus | Georgia | USA | 31.35 | -82.02 | GA11.0032 | |
| B.FR6 | B. fraternus | North Carolina | USA | 34.67 | -77.03 | NC11.0032 | |
| B.FR8 | B. fraternus | North Carolina | USA | 34.67 | -77.03 | NC11.0036 | |
| B.IM10 | IMPATIENS_GA | B. impatiens | Georgia | USA | 31.85 | -82.36 | GA11.0015 |
| B.IM12 | IMPATIENS_GA | B. impatiens | Georgia | USA | 31.85 | -82.36 | GA11.0014 |
| B.IM3 | IMPATIENS_MI | B. impatiens | Michigan | USA | 44.98 | -84.28 | MI11.0028 |
| IMPATIENS_MI | B. impatiens | Michigan | USA | 44.98 | -84.28 | MI11.0031 | |
| B.IM1 | B. impatiens | Michigan | USA | 44.98 | -84.28 | MI11.0030 | |
| B.IM2 | B. impatiens | Michigan | USA | 44.98 | -84.28 | MI11.0029 | |
| B.IM5 | B. impatiens | Michigan | USA | 44.98 | -84.28 | MI11.0032 | |
| B.IM6 | B. impatiens | West Virginia | USA | 37.77 | -80.23 | WV11.0007 | |
| B.IM8 | B. impatiens | West Virginia | USA | 37.77 | -80.23 | WV11.0004 | |
| B.IM9 | B. impatiens | West Virginia | USA | 37.77 | -80.23 | WV11.0006 | |
| B.PE4 | PENSYLVANICUS_FL | B. pensylvanicus | Florida | USA | 29.89 | -82.67 | FL11.0001 |
| PENSYLVANICUS_FL | B. pensylvanicus | Florida | USA | 29.89 | -82.67 | FL11.0003 | |
| B.PE1 | PENSYLVANICUS_NC | B. pensylvanicus | North Carolina | USA | 34.48 | -78.63 | NC11.0042 |
| B.PE5 | PENSYLVANICUS_NC | B. pensylvanicus | North Carolina | USA | 34.48 | -78.63 | NC11.0040 |
| B.PE2 | B. pensylvanicus | Florida | USA | 29.89 | -82.67 | FL11.0002 | |
| B.PE3 | B. pensylvanicus | South Carolina | USA | 34.23 | -79.15 | SC11.0005 | |
| B.PE6 | B. pensylvanicus | South Carolina | USA | 34.23 | -79.15 | SC11.0004 | |
| B.PE9 | B. pensylvanicus | South Carolina | USA | 34.23 | -79.15 | SC11.0003 | |
| B.TRS.7 | TERRESTRIS_ARG | B. terrestris | Río Negro | Argentina | -43.06 | -71.53 | BARI2012.003 |
| B.TRS.8 | TERRESTRIS_ARG | B. terrestris | Río Negro | Argentina | -43.06 | -71.53 | BARI2012.004 |
| B.TRS.1 | TERRESTRIS_FR | B. terrestris | Pyrenees-Orientalis | France | 42.55 | 2.97 | Fr10.350 |
| B.TRS.2 | TERRESTRIS_FR | B. terrestris | Pyrenees-Orientalis | France | 42.55 | 2.97 | Fr10.351 |
| B.TRS.3 | B. terrestris | Pyrenees-Orientalis | France | 42.55 | 2.97 | Fr10.347 | |
| B.TRS.4 | B. terrestris | Pyrenees-Orientalis | France | 42.55 | 2.97 | Fr10.352 | |
| B.TRS.5 | B. terrestris | Río Negro | Argentina | -43.06 | -71.53 | BARI2012.001 | |
| B.TRS.6 | B. terrestris | Río Negro | Argentina | -43.06 | -71.53 | BARI2012.002 | |
| B.TE10 | TERRICOLA_MI | B. terricola | Michigan | USA | 46.16 | -88.77 | MI11.0014 |
| B.TE14 | TERRICOLA_MI | B. terricola | Michigan | USA | 46.16 | -88.77 | MI11.0001 |
| B.TE12 | TERRICOLA_WI | B. terricola | Wisconsin | USA | 45.73 | -89.54 | WI11.0002 |
| B.TE2 | TERRICOLA_WI | B. terricola | Wisconsin | USA | 45.73 | -89.54 | WI11.0009 |
| B.TE13 | B. terricola | Michigan | USA | 46.16 | -88.77 | MI11.0002 | |
| B.TE5 | B. terricola | Michigan | USA | 46.16 | -88.77 | MI11.0018 | |
| B.TE3 | B. terricola | Minnesota | USA | 47.89 | -92.77 | MN11.0043 | |
| B.TE4 | B. terricola | Minnesota | USA | 47.89 | -92.77 | MN11.0027 | |
| B.TE6 | B. terricola | Minnesota | USA | 47.89 | -92.77 | MN11.0026 | |
| B.TE7 | B. terricola | Minnesota | USA | 47.89 | -92.77 | MN11.0038 | |
| B.TE9 | B. terricola | Minnesota | USA | 47.89 | -92.77 | MN11.0040 | |
| B.TE1 | B. terricola | Wisconsin | USA | 45.73 | -89.54 | WI11.0008 | |
| USSURENSIS | B. ussurensis | North Chungcheong | South Korea | 38.1 | 127.07 | ||
| USSURENSIS | B. ussurensis | North Chungcheong | South Korea | 38.1 | 127.07 | ||
| USSURENSIS | B. ussurensis | North Chungcheong | South Korea | 38.1 | 127.07 |
For amplicon sequencing, bumble bees whose DNAs were pooled prior to Roche 454 library preparation were given the same pyrosequencing sample ID.