Table 1. Details of Reference genes and their primer sequences for each of the 11 evaluated genes.
| Gene Symble | Primer sequences (5' to 3') FP/RP | Gene description | Accession Number | Amplicon size (bp) | Tm (°C) | E (%) | Regression coefficiant (R2) |
|---|---|---|---|---|---|---|---|
| 18S-rRNA | TGAGAAACGGCTACCACATC | 18S ribosomal protein | AJ236016.1 | 106 | 62 | 98 | 0.998 |
| AGACTCATAGAGCCCGGTATT | |||||||
| 26S | GAACGGGCTTGGCAGAATTA | 26S ribosomal RNA | JQ408705.1 | 103 | 62 | 97 | 0.996 |
| CCAGCTCCCACCTATACTACAT | |||||||
| ACT | CTTCCCGATGGTCAAGTCATTA | Actin | X55749.1 | 116 | 62 | 96 | 0.997 |
| TTGTATGTGGTCTCGTGGATTC | |||||||
| CYP | AGGTGTTGGAAAGATGGGTAAG | Cyclophilin | AF126551.1 | 87 | 62 | 94 | 0.991 |
| TCACCTCCTTGACACATGAAC | |||||||
| EF-1 | GGAACTTGAGAAGGAGCCTAAG | Elongation factor 1 | X14449.1 | 107 | 62 | 102 | 1.00 |
| ATGGAGGGTATTCAGCAAAGG | |||||||
| GAPDH | GTGCTGACTTCGTTGTTGAATC | Glyceraldehyde-3-phosphate dehydrogenase | U97257.1 | 99 | 62 | 102 | 0.993 |
| GAGCAGAGATCACAACCTTCTT | |||||||
| RPL2 | CCGTCATCCTTTCAGGTACAA | Ribosomal protein L2 | X64562.1 | 119 | 62 | 89 | 0.987 |
| GCAACACGTTACCAACCATAAG | |||||||
| T-SAND | GGTCAATCCGAGTACGAATCC | Sand family protein | SGN-U316474 | 90 | 62 | 90 | 0.995 |
| CGGGAGCTCCTTCAGTTTATC | |||||||
| TUA | CTCTGTGGACTATGGCAAGAAA | Alpha-Tubulin | AJ421411.1 | 101 | 62 | 90 | 0.996 |
| TTGACAGGACACTGTTGTAAGG | |||||||
| TUB | TGGTACACAGGTGAAGGAATG | Beta-tubulin | DQ205342.1 | 102 | 62 | 86 | 0.999 |
| TGCTGTAGCATCCTGGTATTG | |||||||
| UBQ | AGCTGCTATACTGACTTCAATCC | Ubiquitin | AB026056.1 | 111 | 62 | 94 | 0.996 |
Notes: Tm = Annealing temperature, E = PCR efficiency.