Table 3. Ranking orders of the candidate reference genes according to their standard deviation calculated Bestkeeper algorithm.
Rank | Total | Wounding | Drought | Salt | Heat | Cold | Tissue | MJ | SA | ABA | Biotic |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | UBQ | RPL2 | UBQ | EF-1 | T-SAND | 18S-rRNA | CYP | T-SAND | T-SAND | UBQ | TUB |
SD | 0.78 | 0.19 | 0.29 | 0.36 | 0.75 | 0.29 | 0.39 | 0.57 | 0.24 | 0.28 | 0.12 |
2 | RPL2 | CYP | RPL2 | GAPDH | ACT | TUB | EF-1 | RPL2 | UBQ | CYP | RPL2 |
SD | 0.91 | 0.22 | 0.34 | 0.42 | 0.98 | 0.66 | 0.44 | 0.67 | 0.32 | 0.52 | 0.14 |
3 | CYP | 18S-rRNA | 26S | T-SAND | RPL2 | CYP | UBQ | EF-1 | RPL2 | EF-1 | UBQ |
SD | 1.04 | 0.35 | 0.35 | 0.54 | 1 | 0.7 | 0.52 | 0.7 | 0.44 | 0.56 | 0.16 |
4 | EF-1 | 26S | CYP | 26S | 18S-rRNA | UBQ | 26S | CYP | EF-1 | T-SAND | EF-1 |
SD | 1.09 | 0.43 | 0.45 | 0.6 | 1.3 | 0.75 | 0.56 | 0.78 | 0.45 | 0.82 | 0.23 |
5 | T-SAND | UBQ | 18S-rRNA | TUB | 26S | RPL2 | GAPDH | UBQ | 18S-rRNA | TUB | 18S-rRNA |
SD | 1.29 | 0.45 | 0.46 | 0.73 | 1.72 | 0.92 | 0.68 | 0.8 | 0.59 | 0.97 | 0.25 |
6 | 26S | T-SAND | EF-1 | CYP | GAPDH | T-SAND | TUB | TUB | CYP | 26S | ACT |
SD | 1.37 | 0.48 | 0.47 | 0.83 | 2.27 | 0.95 | 0.85 | 0.99 | 0.69 | 0.99 | 0.32 |
7 | TUB | TUB | GAPDH | RPL2 | CYP | EF-1 | RPL2 | 26S | 26S | RPL2 | T-SAND |
SD | 1.48 | 0.53 | 0.59 | 0.93 | 2.35 | 0.96 | 0.93 | 1.06 | 0.71 | 1.01 | 0.38 |
8 | GAPDH | EF-1 | T-SAND | TUA | TUB | 26S | TUA | TUA | TUA | 18S-rRNA | TUA |
SD | 1.62 | 0.65 | 0.63 | 1 | 2.51 | 1.35 | 0.94 | 1.08 | 0.89 | 1.03 | 0.48 |
9 | ACT | ACT | ACT | UBQ | UBQ | ACT | 18S-rRNA | 18S-rRNA | TUB | TUA | 26S |
SD | 1.9 | 0.96 | 0.69 | 1.04 | 2.55 | 1.7 | 1.73 | 1.39 | 1.04 | 1.39 | 0.5 |
10 | TUA | GAPDH | TUB | ACT | EF-1 | GAPDH | ACT | ACT | ACT | ACT | CYP |
SD | 1.91 | 1.02 | 1.04 | 1.36 | 2.65 | 1.71 | 1.84 | 1.43 | 1.73 | 1.83 | 0.64 |
11 | 18S-rRNA | TUA | TUA | 18S-rRNA | TUA | TUA | T-SAND | GAPDH | GAPDH | GAPDH | GAPDH |
SD | 2.06 | 1.56 | 1.45 | 1.61 | 4.45 | 2.09 | 2.65 | 1.53 | 1.8 | 1.9 | 0.97 |
Note. Reference genes with SD values greater than 1 are considered as inconsistent and should be excluded.