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. 2015 Mar 13;10(3):e0118860. doi: 10.1371/journal.pone.0118860

Table 3. Ranking orders of the candidate reference genes according to their standard deviation calculated Bestkeeper algorithm.

Rank Total Wounding Drought Salt Heat Cold Tissue MJ SA ABA Biotic
1 UBQ RPL2 UBQ EF-1 T-SAND 18S-rRNA CYP T-SAND T-SAND UBQ TUB
SD 0.78 0.19 0.29 0.36 0.75 0.29 0.39 0.57 0.24 0.28 0.12
2 RPL2 CYP RPL2 GAPDH ACT TUB EF-1 RPL2 UBQ CYP RPL2
SD 0.91 0.22 0.34 0.42 0.98 0.66 0.44 0.67 0.32 0.52 0.14
3 CYP 18S-rRNA 26S T-SAND RPL2 CYP UBQ EF-1 RPL2 EF-1 UBQ
SD 1.04 0.35 0.35 0.54 1 0.7 0.52 0.7 0.44 0.56 0.16
4 EF-1 26S CYP 26S 18S-rRNA UBQ 26S CYP EF-1 T-SAND EF-1
SD 1.09 0.43 0.45 0.6 1.3 0.75 0.56 0.78 0.45 0.82 0.23
5 T-SAND UBQ 18S-rRNA TUB 26S RPL2 GAPDH UBQ 18S-rRNA TUB 18S-rRNA
SD 1.29 0.45 0.46 0.73 1.72 0.92 0.68 0.8 0.59 0.97 0.25
6 26S T-SAND EF-1 CYP GAPDH T-SAND TUB TUB CYP 26S ACT
SD 1.37 0.48 0.47 0.83 2.27 0.95 0.85 0.99 0.69 0.99 0.32
7 TUB TUB GAPDH RPL2 CYP EF-1 RPL2 26S 26S RPL2 T-SAND
SD 1.48 0.53 0.59 0.93 2.35 0.96 0.93 1.06 0.71 1.01 0.38
8 GAPDH EF-1 T-SAND TUA TUB 26S TUA TUA TUA 18S-rRNA TUA
SD 1.62 0.65 0.63 1 2.51 1.35 0.94 1.08 0.89 1.03 0.48
9 ACT ACT ACT UBQ UBQ ACT 18S-rRNA 18S-rRNA TUB TUA 26S
SD 1.9 0.96 0.69 1.04 2.55 1.7 1.73 1.39 1.04 1.39 0.5
10 TUA GAPDH TUB ACT EF-1 GAPDH ACT ACT ACT ACT CYP
SD 1.91 1.02 1.04 1.36 2.65 1.71 1.84 1.43 1.73 1.83 0.64
11 18S-rRNA TUA TUA 18S-rRNA TUA TUA T-SAND GAPDH GAPDH GAPDH GAPDH
SD 2.06 1.56 1.45 1.61 4.45 2.09 2.65 1.53 1.8 1.9 0.97

Note. Reference genes with SD values greater than 1 are considered as inconsistent and should be excluded.