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. 2015 Mar 13;10(3):e0118860. doi: 10.1371/journal.pone.0118860

Table 5. Relative stability ranking of potential reference genes based on geNorm, NormFinder, Bestkeeper and ΔCt method.

Experimental conditions Four most stable genes Recommended comprehensive ranking Two Least stable genes
geNorm NormFinder Bestkeeper ΔCt
All CYP,EF-1, UBQ,RPL2 TUB, CYP, UBQ, 26S UBQ, RPL2, CYP,EF-1 CYP, EF-1, UBQ, RPL2 CYP, EF-1,UBQ,RPL2 18S-rRNA, TUA
Wounding CYP, UBQ, TUB, RPL2 CYP, RPL2, TUB, 18S-rRNA RPL2, CYP, 18S-rRNA, 26S CYP, RPL2, TUB, 18S-rRNA CYP, RPL2, TUB, 18S-rRNA TUA, GAPDH
Salt TUB, RPL2, CYP,T-SAND UBQ, GAPDH, CYP, EF-1 EF-1,GAPDH,T-SAND, 26S T-SAND, CYP,TUB,RPL2 T-SAND,TUB, CYP,EF-1 18S-rRNA, ACT
Drought 26S, RPL2, UBQ, CYP 26S, UBQ, RPL2, ACT UBQ, RPL2, 26S, CYP 26S, UBQ, RPL2, CYP 26S, UBQ, RPL2, CYP TUA, TUB
Heat CYP, EF-1, TUB, 26S 26S, TUB, CYP, GAPDH T-SAND, ACT, RPL2, 18S-rRNA CYP, TUB, 26S, EF-1 CYP, 26S, TUB, EF-1 TUA, 18S-rRNA
Cold CYP, UBQ, TUB, RPL2 TUB, RPL2, EF-1, T-SAND 18S-rRNA, TUB, CYP, UBQ TUB, RPL2, EF-1, UBQ TUB, UBQ, RPL2, CYP TUA, ACT
Tissue CYP, EF-1, GAPDH,RPL2 CYP, RPL2 GAPDH, EF-1 CYP, EF-1, UBQ, 26S CYP,EF-1,GAPDH, RPL2 CYP,EF-1,GAPDH,RPL2 T-SAND, ACT
MJ CYP, TUB, T-SAND, 18S-rRNA CYP, T-SAND, TUB, TUA T-SAND, RPL2, EF-1, CYP CYP, T-SAND, TUB, TUA CYP, T-SAND, TUB, RPL2 26S, UBQ
SA T-SAND, UBQ, RPL2, EF-1 T-SAND, CYP, 18S-rRNA, RPL2 T-SAND, UBQ, RPL2, EF-1 T-SAND, CYP, 18S-rRNA, RPL2 T-SAND, UBQ, RPL2, CYP GAPDH, ACT
ABA RPL2, EF-1, 26S, UBQ 18S-rRNA, T-SAND, TUB, CYP UBQ, CYP, EF-1, T-SAND 18S-rRNA, T-SAND, TUB, EF-1 18S-rRNA, EF-1, T-SAND, UBQ GAPDH, ACT
Biotic UBQ, T-SAND, EF-1, 18S-rRNA UBQ, T-SAND, EF-1,CYP TUB, RPL2,UBQ,EF-1 EF-1, CYP, T-SAND, UBQ UBQ, T-SAND, EF-1, TUB GAPDH, TUA