Figure 8. Volatile oil-induced NO production is blocked by BAPTA-AM.

Cultured HUVECs were pre-treated with serum free medium or BAPTA-AM (5 µM) for 3 hours, and then labeled with fluorescent NO indicator DAF-FM DA for 30 min. Fluorimetric measurement was performed after the treatment of NRR volatile oil (25 µg/mL), A23187 (1 µM, positive control). The amounts of NO were evaluated by measuring the fluorescence intensity. Micrographs were taken by the confocal microscope. Bar = 100 µm (upper panel). Quantification of NO production was displayed as a ratio of fluorescence intensity at 10 min (F10) to the control at time 0 (F0) in the cultures (lower panel). Mean ± SEM, n = 3, each with triplicate samples. **p<0.01.