Figure 1. Effects of cisplatin on proliferation and colony formation in triple-negative breast cancer (TNBC) cells.

(A) CXCR4 knockdown and CXCR4 overexpression cells were treated with cisplatin for 48 h. The effect of CXCR4 on cisplatin sensitivity was measured by MTT assay. (B) IC₅₀ values for cisplatin in MDA-MB-231 and MDA-MB-468 cells for 48 h were calculated by regression analysis using SPSS software based on the results of the MTT assays. (C) Colony formation of MDA-MB-231-NC and MDA-MB-231-shCXCR4 cells following treatment with cisplatin (0, 0.1 and 1 μM) for 48 h. (D) Colony formation of MDA-MB-468-NC and MDA-MB-468-CXCR4 cells following treatment with cisplatin (0, 0.1 and 1 μM) for 48 h. (E) The percentage of colony formation of MDA-MB-231 and MDA-MB-468 cells treatment with cisplatin. Data are represented as the mean ± SD of triplicate determinations. Each assay was performed in triplicate and repeated at least three times. *p < 0.05, **p < 0.01, as compared with untreated cells.