Skip to main content
. 2014 Dec 11;6(2):1020–1030. doi: 10.18632/oncotarget.2741

Figure 4. The effect of CXCR4 on protein expression and cell cycle associated proteins in TNBC cells.

Figure 4

(A) MDA-MB-231-NC and MDA-MB-231-shCXCR4 cells were treated with cisplatin (0, 10 μM) for 48 h, cellular protein was harvested, and Western blot analysis was performed to investigate the levels of mutant p53, Bax, Bcl-2 and caspase-3. The β-Actin was detected as a loading control. (B) MDA-MB-468-NC and MDA-MB-468-CXCR4 cells were treated with cisplatin (0, 10 μM) for 48 h, cellular protein was harvested, and Western blot analysis was performed to investigate the levels of mutant p53, Bax, Bcl-2 and caspase-3. The β-Actin was detected as a loading control. (C) Immunohistochemical staining of xenograft tumors for p53, Bax, Bcl-2 and caspase-3 (magnification = 40×).