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. 2014 Nov 26;6(2):1079–1089. doi: 10.18632/oncotarget.2835

Figure 4. Expression of E1a and Hsp70 mediated by oncolytic adenoviruses.

Figure 4

(A) Tumor xenografts were fixed in 10% neutralized formalin for 6 h to prepare the paraffin-embedded sections. SP immunohistochemical staining was performed to locate the expressions of indicated proteins; original magnification: ×200. (B) For each slice, the percentages of positive cells were counted within 5 medium-power magnification fields of view (20× objective lens) under microscope. The results were determined by scoring the stained cell ratio and the staining intensity; ***P<0.001. (C) Mouse blood was collected to prepare sera. The expression of Hsp70 protein was detected using an ELISA; ***P<0.001.