Figure 4. TGFβ1 altered EMT with enhanced PCa invasion via modulating CD44.

(a) After 5ng/ml TGFβ₁ treatment, the decrease expression of E-Cadherin and the increase expression of vimentin were detected in LNCaP and CWR22rv1 cells in a time dependent manner by Western blot. (b) EMT transition induced by TGFβ₁ could be blocked by CD44 siRNA in LNCaP and CWR22rv1 cells. LNCaP and CWR22rv1 cells were treated with 5ng/ml TGFβ₁ with or without CD44 siRNA for 12hrs and 24hrs. The expressions of CD44, E-Cadherin and Vimentin of different treatments were detected by Western blot assay. (c) Invasion ability of LNCaP and CWR22rv1 cells treated with TGFβ₁ (5ng/ml), CD44 siRNA and both treatments were analyzed in Transwell Chamber assay. Quantitation was shown on the right. Significance was defined as p<0.05(*).