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. 2014 Nov 26;6(2):1249–1261. doi: 10.18632/oncotarget.2859

Figure 4. RBP2-mediated differentiation and proliferation depends in part on miR-21 in K562 and HL60 cells.

Figure 4

(A, B) qRT-PCR analysis of level of miR-21 in differentiated cells induced by DMSO or ATRA. Data are mean ± SEM of 3 independent experiments. (C, D) Cell proliferation of K562 and HL60 cells after transfection with miR-21 inhibitor. (E, F) Foci formation of K562 and HL60 cells after transfection with miR-21 inhibitor. (G) qRT-PCR analysis of miR-21 and the loading control U6 snRNA 48 h after RBP2 expression plasmid transfection without or with miR-21 mimics. Data are mean ± SEM 3 of independent experiments. (H, I) Cell proliferation assay of K562 and HL60 cells after RBP2 expression plasmid transfection without or with miR-21 mimics for 24 and 48 h. (J, K) Colony-formation assay of K562 and HL60 cells after RBP2 expression plasmid transfection without or with miR-21 mimics for 48 h. The results are from 3 independent experiments. *P< 0.05, **P< 0.01, ***P < 0.001.