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. 2014 Nov 26;6(2):1249–1261. doi: 10.18632/oncotarget.2859

Figure 5. PDCD4 is directly downregulated by miR-21.

Figure 5

qRT-PCR analysis of the level of (A) mature miR-21 and (B) PDCD4 after transfection with miR-21 mimics or inhibitor. Data are mean ± SEM of 3 independent experiments. (C) Western blot analysis of PDCD4 protein level with miR-21 mimics or inhibitor treatment. β-actin was a loading control. qRT-PCR analysis of the mRNA level of (D) RBP2 and (E) PDCD4 with RBP2 expression plasmid transfection without or with miR-21 mimics. Data are mean ± SEM of 3 independent experiments. (F) Western blot analysis of RBP2 and PDCD4 protein level with RBP2 expression plasmid transfection without or with miR-21 mimics. β-actin was a loading control. The results are from 3 independent experiments. *P< 0.05, **P< 0.01, ***P < 0.001.