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. 2014 Dec 1;6(2):1302–1314. doi: 10.18632/oncotarget.2744

Figure 2. AMACR detection using SmartFlares in human prostate cancer needle biopsy.

Figure 2

(A) upper panel: Specific AMACR-Cy5 signal in EPCAM positive cells (unstained and scramble-Cy5 controls); lower panel: gating strategy for size exclusion, singlets, living cells and EPCAM positive cells; (B) Enrichment of AMACR-CY5 positive cells in high gate compared to scramble-Cy5 and benign tissue; (C) AMACR-high expressing cells express significant levels of tumor markers and decreased levels of basal cell markers p63 and cytokeratin 5 (KRT5); (D) 1000 epithelial cells were expanded for several days with typical morphology (upper panel: 10x, lower panel 20x) in PREGM supplemented with 15% Matrigel; bar = 100μm.