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. 2014 Dec 15;6(2):617–632. doi: 10.18632/oncotarget.2710

Figure 2. G2E3 depletion induces p53-dependent accumulation of p21.

Figure 2

(A) G2E3 depletion enhances the levels of p21. U2OS cells were transfected with combinations of siRNAs targeting G2E3 and Mdm2 as indicated. Knockdown of p53 served as a control. After 48 h, the cells were harvested and analyzed by immunoblotting and detection using antibodies to the indicated proteins. (B) G2E3 knockdown induces CDKN1A/p21 mRNA accumulation. U2OS cells were depleted of G2E3, p21 and p53 by siRNA-mediated knockdown. After 64 h, the cells were harvested and CDKN1A/p21 mRNA levels were analyzed by quantitative RT-PCR, in relation to the reference gene GAPDH. Data are represented as mean. Error bars represent the standard deviation (SD, n = 3). *p < 0.05, **p < 0.01, n.s. = not significant (Student's t-test). (C) p21 induction by G2E3 depletion depends on p53. U2OS cells were transfected with combinations of siRNAs targeting G2E3 and p53 as indicated. Knockdown of Mdm2 served as control. After 64 h, cells were harvested and analyzed as in A.