Skip to main content
. 2014 Dec 15;6(2):617–632. doi: 10.18632/oncotarget.2710

Figure 4. Knockdown of G2E3 results in p53-independent apoptosis and chemosensitization of osteosarcoma and colon carcinoma cells.

Figure 4

U2OS (A), HCT116 p53+/+ (B) and HCT116 p53−/− (C) cells were depleted of G2E3 by siRNA-mediated knockdown and either left untreated or treated with 30 μM cisplatin for 16 h. Cell lysates were analyzed by immunoblotting. Markers for apoptosis are cleaved caspase 3 (Cleaved Casp. 3), in comparison with total caspase 3, and cleaved PARP-1 (full-length protein at 115 kDa and cleaved PARP-1 at 90 kDa).