Figure 4. Proximal stomach signaling mechanisms in 12 week old gp130^757FF xIL-1RT1^−/− mice and, wildtype and IL-1RT1^−/− mice administered 1ug of IL-11 daily for 7 days. gp130^757FF mice develop gastric pathology because of hyperactivation of STAT3; therefore the expression of gp130 ligands and activations of downstream transcription factors were analyzed.

(A) Immunoblot analysis of STAT3, ERK1/2 and AKT phosphorylation in 12 week old gp130^757FF xIL-1RT1^−/− mice and expression of (B) IL-6 mRNA and (C) IL-11 (i) mRNA and protein (ii). (D) Immunoblot analysis of STAT3, ERK1/2 and AKT phosphorylation in wildtype and IL-1RT1^−/− mice administered 1μg of IL-11 for 7 days and mRNA expression of (E) IL-6, and (F) IL-11. Bars are means ± SEM, p-values are presented for statistically significant changes (p < 0.05).