Skip to main content
. 2014 Dec 23;6(2):771–788. doi: 10.18632/oncotarget.2718

Figure 6. SKP2 positively correlates with H3K4me3 in human prostate cancer specimens.

Figure 6

(A) Immunohistochemical staining on SKP2 and H3K4me3 in human prostate array tissues. Scale bars represent 50 μm. (B) Statistical analysis of the prostate tissue microarray stained with SKP2 and H3K4me3 antibodies. The percentages of different H3K4me3 levels were calculated for each level of SKP2 protein in 35 cases of human PCa specimens. SKP2 and H3K4me3 levels were graded as 0, 1, 2 and 3 by intensity scores. The H3K4me3 grades are color-coded. Numbers in parenthesis represent sample sizes. The statistical significance was determined by Chi-Square test (Table S2). (C) A working model for the role of SKP2 on epigenetic regulation of JARID1B and H3K4me3 in PCa. SKP2 suppresses the activity of JARID1B by reducing its K63-linked ubiquitination through TRAF6 under oncogenic stimulation, leading to an elevated H3K4me3 thus contributes to PCa. SKP2 deficiency increases JARID1B transportation to nucleolus of cells through an increase of its ubiquitination, resulting in an induction of cellular senescence to suppress PCa tumorigenesis.